l ala gln (Beyotime)

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Beyotime l ala gln
L Ala Gln, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/l+ala+gln/L-Ala-Gln/pmc12903623-113-42-43
Average 99 stars, based on 10 article reviews

l ala gln - by Bioz Stars, 2026-09

99/100 stars

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Article Title: USP24 promotes autophagy-dependent ferroptosis in hepatocellular carcinoma by reducing the K48-linked ubiquitination of Beclin1
Article Snippet: .. Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Solarbio, Beijing, China) supplemented with 10% fetal bovine serum (FBS, AC03L055, Shanghai Life-iLab Biotech, China), 1% penicillin-streptomycin and 1% L-Ala-Gln (Beyotime, Jiangsu, China). .. All cell lines mentioned above were cultured in a humidified incubator at 37 °C with 5% CO . For transfection, three small interfering RNAs targeting USP24 (si-USP24-1, si-USP24-2, si-USP24-3) and the negative control (si-NC), one small interfering RNA targeting Beclin1 (si-Beclin1) and its negative control were acquired from RiboBio (Guangzhou, China).

Article Title: Tight Junction Component Occludin Binds to FIP5 to Regulate Endosome Trafficking and Mitotic Spindle Function.
Article Snippet: The cell cycle and apoptosis analysis kit (YEASEN, #40301) was used to analyze the cell cycle, and FlowJo was used for cell cycle analysis. .. Cell Culture: HC11 cells were cultured in RPMI 1640 (Gibco, #C11875500CP), supplemented with 10% FBS, 5 μg mL−1 insulin, 10 ng mL−1 EGF (GenScript, #Z02691), 1% L-Ala-Gln (Beyotime, #C0211), and 1% P/S. .. HEK293T cells were cultured in DMEM (Gibco, #C12430500BT), supplemented with 10% FBS, 1% sodium pyruvate (Beyotime, #C0331), 1% L-Ala-Gln, 1% non-essential amino acids (Beyotime, #C0332), and 1% P/S.

Article Title: Visualizing bulk autophagy in vivo by tagging endogenous LC3B.
Article Snippet: Macroautophagy/autophagy plays a crucial role in maintaining cellular and organismal health, making the measurement of autophagy flux in vivo essential for its study.. Current tools often depend on the overexpression of autophagy probes.. In this study, we developed a knock-in mouse model, termed tfLC3-KI, by inserting a tandem fluorescent tag coding sequence into the native Map1lc3b gene locus.

Article Title: USP24 promotes autophagy-dependent ferroptosis in hepatocellular carcinoma by reducing the K48-linked ubiquitination of Beclin1.
Article Snippet: .. Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Solarbio, Beijing, China) supplemented with 10% fetal bovine serum (FBS, AC03L055, Shanghai Life-iLab Biotech, China), 1% penicillin-streptomycin and 1% L-Ala-Gln (Beyotime, Jiangsu, China). ..

Article Title: Tight Junction Component Occludin Binds to FIP5 to Regulate Endosome Trafficking and Mitotic Spindle Function
Article Snippet: HC11 cells were cultured in RPMI 1640 (Gibco, #C11875500CP), supplemented with 10% FBS, 5 μg mL −1 insulin, 10 ng mL −1 EGF (GenScript, #Z02691), 1% L‐Ala‐Gln (Beyotime, #C0211), and 1% P/S. .. HEK293T cells were cultured in DMEM (Gibco, #C12430500BT), supplemented with 10% FBS, 1% sodium pyruvate (Beyotime, #C0331), 1% L‐Ala‐Gln, 1% non‐essential amino acids (Beyotime, #C0332), and 1% P/S. .. Primary luminal cells were cultured in advanced DMEM/F12 (Gibco, #12 634 010) supplemented with 5% FBS (Gibco, #10099141), 10 ng mL −1 EGF, 20 ng mL −1 FGF2 (GenScript, #Z03116), 5 μM Y27632 (Sellect, S1049), 4 μg mL −1 Heparin (Thermo, #A16198), 1% sodium pyruvate, 1% L‐Ala‐Gln, 1% P/S, and 5% Matrigel (Corning, #354234).

Article Title: Macrophage extracellular traps as key mediators of scleral remodeling in myopia induced by hypoxia and activated platelets.
Article Snippet: .. HFSF were cultured in DMEM (11965092, Gibco, USA) with 10% non-heat-inactivated FBS (10099141C, Gibco, USA), 2 mM L-Ala-Gln (C0211, Beyotime, China), 100 U/mL penicillin, and 100 μg/mL streptomycin (15140122, Gibco, USA) at 37◦C under a 5% CO2 atmosphere. ..

Article Title: Tight Junction Component Occludin Binds to FIP5 to Regulate Endosome Trafficking and Mitotic Spindle Function
Article Snippet: The cell cycle and apoptosis analysis kit (YEASEN, #40301) was used to analyze the cell cycle, and FlowJo was used for cell cycle analysis. .. HC11 cells were cultured in RPMI 1640 (Gibco, #C11875500CP), supplemented with 10% FBS, 5 μg mL −1 insulin, 10 ng mL −1 EGF (GenScript, #Z02691), 1% L‐Ala‐Gln (Beyotime, #C0211), and 1% P/S. .. HEK293T cells were cultured in DMEM (Gibco, #C12430500BT), supplemented with 10% FBS, 1% sodium pyruvate (Beyotime, #C0331), 1% L‐Ala‐Gln, 1% non‐essential amino acids (Beyotime, #C0332), and 1% P/S.

Article Title: Single-cell multi-omics reveals DUSP9 as a key regulator of cancer stemness and a potential therapeutic target in hepatocellular carcinoma
Article Snippet: .. The human HCC cells HepG2 and Huh7 were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China), and cultured in DMEM (Solarbio, Beijing, China) supplemented with 10% fetal bovine serum (Shanghai Life-iLab Biotech, China), 1% penicillin-streptomycin and 1% L-Ala-Gln (Beyotime, Jiangsu, China). .. All cell lines mentioned above were cultured in a humidified incubator at 37 °C with 5% CO . For transfection, three small interfering RNAs targeting DUSP9 and the negative control (siNC) were acquired from RiboBio (Guangzhou, China).

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